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UEFA have also banished Barcelona midfielder Thiago Motta's appeal.
Douglas was involved in a half time scuffle in Celtic Park's tunnel, in the first leg of the Fourth Round tie. Douglas still protests his innocence, and his case has also been backed up by several Barca players.
However, UEFA stuck by the original decision made, and handed out a one match ban to each player.
They also said there will be no investigation into Bobo Balde's involvement in the incident.
David Marshall will start in Nou Camp.